应用as-pcr检测牛尿苷酸合酶缺乏症的方法

Method for detecting bovine uridine monophosphate synthase deficiency disease by using AS-PCR

Abstract

本发明属于动物疾病检测技术领域,具体涉及应用AS-PCR检测牛尿苷酸合酶缺乏症的方法。制备了一种用于牛尿苷酸合酶缺乏症检测方法的特异性PCR引物,它包括:UMPS基因扩增引物:突变型:反向引物3’端倒数第三位引入错配,扩增产物长度为261bp;1F:5’ATGGGAGAAGGGGATAGGAG 3’,1R:5’TCTGGTTTCATGCTTACGCA3’;野生型:正向引物3’倒数第三位引入错配产物,长度90bp;2F:5’ATTGGTTTTATTTCTGGCTACC 3’,2R:5’AGGCTTACCTCCTGCTTCTA 3’。本发明制备的牛尿苷酸合酶缺乏症的方法的特异性引物,可直接应用于开展牛尿苷酸合酶缺乏症的致病基因的筛选,以利于对患病个体进行早期淘汰。
The invention belongs to the technical field of animal disease detection, and particularly relates to a method for detecting bovine uridine monophosphate synthase deficiency disease by using AS-PCR. A specific PCR primer is prepared for the method for detecting the bovine uridine monophosphate synthase deficiency disease, and comprises UMPS gene amplification primers including: a mutant primer, wherein the last but two position introduction of 3'end of a reverse primer is mismatched, and the length of an amplified product is 261bp; 1F of 5'ATGGGAGAAGGGGATAGGAG 3', and 1R of 5'TCTGGTTTCATGCTTACGCA3'; a wild type primer, wherein the last but two position of a forward primer 3' is introduced with a mismatched product with the length of 90bp; and 2F of 5'ATTGGTTTTATTTCTGGCTACC 3', and 2R of 5'AGGCTTACCTCCTGCTTCTA 3'. The specific primer prepared for the method for detecting the bovine uridine monophosphate synthase deficiency disease can be directly used for screening morbigenous genes ofthe bovine uridine monophosphate synthase deficiency disease, and contributes to performing early elimination on sick individuals.

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Cited By (1)

    Publication numberPublication dateAssigneeTitle
    CN-103627804-AMarch 12, 2014华南农业大学一种牛尿苷酸合酶缺乏症有害基因检测的引物组合物及其试剂盒和应用