一种液相色谱柱混合填料及色谱柱

Mixed fillers of liquid-phase chromatographic column and chromatographic column

Abstract

The invention relates to mixed fillers of a liquid-phase chromatographic column and the chromatographic column, and relates to the technical field of analytical chemistry. The mixed fillers of the liquid-phase chromatographic column comprise long-chain alkyl dimethyl silane bonded silica gel and acylamino silane bonded silica gel in a weight ratio of 10:1-1:10, wherein the difference of the mean particle diameter is in a range of between -20 and 20 percent and the difference of the specific weight is in a range of between -20 and 20 percent. In the chromatographic column made by the mixed fillers, the mixed fillers are filled in a column pipe; the outer sides of the two ends of the column pipe are connected with a pipeline through internal thread; the inner sides of the two ends of the column pipe are provided with sieve plates used for preventing the mixed fillers from leaking outwards; one end of the column pipe is provided with a liquid inlet; and a mobile phase enters the chromatographic column from the liquid inlet. The characteristics of the mixed fillers of the liquid-phase chromatographic column are applied to chromatographic separation under the condition of the mixed mobile phase containing acetonitrile and aqueous solution, wherein the separation application is the chromatographic separation and detection of a sample containing a compound with extremely strong hydrophilism and a compound with extremely strong lipotropism simultaneously; and the separation detection of all the components can be performed once under the condition of equal-grade liquid-phase chromatogram.
一种液相色谱柱混合填料及色谱柱,涉及分析化学技术领域,液相色谱柱混合填料包括长链烷基二甲基硅烷键合硅胶和酰胺基硅烷键合硅胶,其重量比为10∶1-1∶10,平均粒径相差在±20%以内,比重相差在±20%以内,一种由混合填料制成的色谱柱,柱管内填充混合填料,柱管两端外侧有内罗纹与管路相连,两端内侧有筛板防止混合填料外漏,柱管的一端设有进液孔,流动相从进液孔进入色谱柱,本发明的特征应用为在含有乙腈和水溶液混合流动相条件下的色谱分离,分离应用是同时含有亲水性极强的化合物和亲脂性极强的化合物的样品的色谱分离和检测,在等度液相色谱条件下,可一次分离完成所有组分的分离检测。

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Patent Citations (2)

    Publication numberPublication dateAssigneeTitle
    CN-1759931-AApril 19, 2006安捷伦科技有限公司用在高效液相色谱中的新型固定相
    CN-1943848-AApril 11, 2007天津博纳固体材料科技有限公司Hydrophilic forward chromatographic column, filler and separating method

NO-Patent Citations (1)

    Title
    JP特开2002-40007A 2002.02.06

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