The present invention provides a high-efficiency extracting method of food-borne pathogen nucleic acid. A PCR technology has been widely used for the detection of pathogenic microorganisms currently. A first step of the PCR detecting technology is the preparation of template nucleic acid, that is, the extraction of nucleic acid in a sample, which directly affects the result of PCR reaction. For along time, the processes of extraction and purification of the nucleic acid in a sample consume time, and are fussy all the time, so detection speed is seriously slowed down. Thus, a plurality of scholars searches after the extracting method of various nucleic acids. The high-efficiency extracting method of food-borne pathogen nucleic acid comprises the following steps: bacterial culture solution is centrifuged; buffer solution A is added into centrifugal sediment to be vibrated, and muramidase is added; prolease K is added after incubation; buffer solution B is added to be incubated at the temperature of 65 DEG C for 10 minutes after being vibrated violently to be prepared into cracking solution; and high-quality nucleic acid is collected by an adsorption film of silicon matrix SiO2 material is used for extracting nucleic acid solution. The present invention is applied to the detection of the pathogenic microorganisms.