Method for culturing fermentation seed liquid by adopting solid straws as carbon source

一种以固体秸秆为碳源培养发酵种子液的方法

Abstract

本发明涉及一种以固体秸秆为碳源培养发酵种子液的方法,其具体步骤为,(1)发酵菌种的活化:从菌种保藏平板、保藏斜面或冷冻甘油保藏管保存的菌种接种于合成培养基;(2)培养基中添加预处理的固体秸秆进行同步糖化驯化培养种子;(3)种子液的多级扩大培养过程;(4)接种与发酵:将步骤(2)或(3)培养获得的含发酵菌种的浆液接种于高固含量的发酵罐进行同步糖化发酵制备乙醇或其他生物基产品。本发明的优点:消除了固液分离获得水解液清液过程中的染菌风险,提高了菌株对抑制物和固体基质的耐受性,过程成本降低,发酵周期缩短,具有很好的工业化应用前景。
The invention relates to a method for culturing fermentation seed liquid by adopting solid straws as a carbon source. The method comprises the specific steps: (1) activation of fermentation strains: strains stored from strain preservation plates, preservation slants or frozen glycerin preservation tubes are inoculated in synthetic medium; (2) pretreated solid straws are added into the medium to carry out synchronous saccharification and naturalization for culturing the seeds; (3) process of the multi-stage intermediate culture of seed liquid; and (4) inoculation and fermentation: the serous fluid which is obtained by the culture of steps (2) and (3) and contains fermentation strains is inoculated into a fermentation tank with high solid content to carry out synchronous saccharification and fermentation to prepare alcohol or other biological base products. The method has the advantages of: eliminating microbiological contamination risk in the process of obtaining clear liquid of hydrolysis liquid by solid-liquid separation, improving the tolerance of strains on the inhibitory substances and solid matrix, and having low process cost, short fermentation period and very good industrialized application prospect.

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Patent Citations (2)

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NO-Patent Citations (1)

    Title
    武秀琴等.玉米秸秆发酵生产燃料酒精研究现状及前景.《现代农业科技》.2008,(第13期),第240-241,243页.

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